Uncovering the Number and Clonal Dynamics of Mesp1 Progenitors during Heart Morphogenesis.
Cell Rep
; 14(1): 1-10, 2016 Jan 05.
Article
em En
| MEDLINE
| ID: mdl-26725109
The heart arises from distinct sources of cardiac progenitors that independently express Mesp1 during gastrulation. The precise number of Mesp1 progenitors that are specified during the early stage of gastrulation, and their clonal behavior during heart morphogenesis, is currently unknown. Here, we used clonal and mosaic tracing of Mesp1-expressing cells combined with quantitative biophysical analysis of the clonal data to define the number of cardiac progenitors and their mode of growth during heart development. Our data indicate that the myocardial layer of the heart derive from â¼250 Mesp1-expressing cardiac progenitors born during gastrulation. Despite arising at different time points and contributing to different heart regions, the temporally distinct cardiac progenitors present very similar clonal dynamics. These results provide insights into the number of cardiac progenitors and their mode of growth and open up avenues to decipher the clonal dynamics of progenitors in other organs and tissues.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica no Desenvolvimento
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Organogênese
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Fatores de Transcrição Hélice-Alça-Hélice Básicos
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Coração
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article