Selection of Color-Changing and Intensity-Increasing Fluorogenic Probe as Protein-Specific Indicator Obtained via the 10BASE(d)-T.
Anal Chem
; 88(2): 1096-9, 2016 Jan 19.
Article
em En
| MEDLINE
| ID: mdl-26727351
ABSTRACT
To obtain a molecular probe for specific protein detection, we have synthesized fluorogenic probe library of vast diversity on bacteriophage T7 via the gp10 based-thioetherificaion (10BASE(d)-T). A remarkable color-changing and turning-on probe was selected from the library, and its physicochemical properties upon target-specific binding were obtained. Combination analyses of fluorescence emission titration, isothermal titration calorimetry (ITC), and quantitative saturation-transfer difference (STD) NMR measurements, followed by in silico docking simulation, rationalized the most plausible geometry of the ligand-protein interaction.
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Base de dados:
MEDLINE
Assunto principal:
Compostos de Sulfidrila
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Proteínas Virais
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Sondas Moleculares
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Bacteriófago T7
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Cor
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Éteres
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Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article