RNA-Seq identifies SPGs as a ventral skeletal patterning cue in sea urchins.
Development
; 143(4): 703-14, 2016 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-26755701
ABSTRACT
The sea urchin larval skeleton offers a simple model for formation of developmental patterns. The calcium carbonate skeleton is secreted by primary mesenchyme cells (PMCs) in response to largely unknown patterning cues expressed by the ectoderm. To discover novel ectodermal cues, we performed an unbiased RNA-Seq-based screen and functionally tested candidates; we thereby identified several novel skeletal patterning cues. Among these, we show that SLC26a2/7 is a ventrally expressed sulfate transporter that promotes a ventral accumulation of sulfated proteoglycans, which is required for ventral PMC positioning and skeletal patterning. We show that the effects of SLC perturbation are mimicked by manipulation of either external sulfate levels or proteoglycan sulfation. These results identify novel skeletal patterning genes and demonstrate that ventral proteoglycan sulfation serves as a positional cue for sea urchin skeletal patterning.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteoglicanas
/
Ouriços-do-Mar
/
Sulfatos
/
Análise de Sequência de RNA
/
Padronização Corporal
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article