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Differential protein expression and basal lamina remodeling in human heart failure.
Kim, Evelyn H; Galchev, Vladimir I; Kim, Jin Young; Misek, Sean A; Stevenson, Tamara K; Campbell, Matthew D; Pagani, Francis D; Day, Sharlene M; Johnson, T Craig; Washburn, Joseph G; Vikstrom, Karen L; Michele, Daniel E; Misek, David E; Westfall, Margaret V.
Afiliação
  • Kim EH; Department of Surgery, University of Michigan, Ann Arbor, MI, USA.
  • Galchev VI; Department of Surgery, University of Michigan, Ann Arbor, MI, USA.
  • Kim JY; Korea Basic Science Institute, Ochang, Korea.
  • Misek SA; Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI, USA.
  • Stevenson TK; Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI, USA.
  • Campbell MD; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
  • Pagani FD; Department of Cardiac Surgery, University of Michigan, Ann Arbor, MI, USA.
  • Day SM; Cardiovascular Division, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
  • Johnson TC; DNA Sequencing and Microarray Facility, University of Michigan, Ann Arbor, MI, USA.
  • Washburn JG; DNA Sequencing and Microarray Facility, University of Michigan, Ann Arbor, MI, USA.
  • Vikstrom KL; Cardiovascular Division, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
  • Michele DE; Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI, USA.
  • Misek DE; Cardiovascular Division, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
  • Westfall MV; Department of Surgery, University of Michigan, Ann Arbor, MI, USA.
Proteomics Clin Appl ; 10(5): 585-96, 2016 05.
Article em En | MEDLINE | ID: mdl-26756417
ABSTRACT

PURPOSE:

A goal of this study was to identify and investigate previously unrecognized components of the remodeling process in the progression to heart failure by comparing protein expression in ischemic failing (F) and nonfailing (NF) human hearts. EXPERIMENTAL

DESIGN:

Protein expression differences were investigated using multidimensional protein identification and validated by Western analysis. This approach detected basal lamina (BL) remodeling, and further studies analyzed samples for evidence of structural BL remodeling. A rat model of pressure overload (PO) was studied to determine whether nonischemic stressors also produce BL remodeling and impact cellular adhesion.

RESULTS:

Differential protein expression of collagen IV, laminin α2, and nidogen-1 indicated BL remodeling develops in F versus NF hearts Periodic disruption of cardiac myocyte BL accompanied this process in F, but not NF heart. The rat PO myocardium also developed BL remodeling and compromised myocyte adhesion compared to sham controls. CONCLUSIONS AND CLINICAL RELEVANCE Differential protein expression and evidence of structural and functional BL alterations develop during heart failure. The compromised adhesion associated with this remodeling indicates a high potential for dysfunctional cellular integrity and tethering in failing myocytes. Therapeutically targeting BL remodeling could slow or prevent the progression of heart disease.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Membrana Basal / Glicoproteínas de Membrana / Laminina / Isquemia Miocárdica / Colágeno Tipo IV / Insuficiência Cardíaca Tipo de estudo: Prognostic_studies Limite: Aged / Animals / Humans / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Membrana Basal / Glicoproteínas de Membrana / Laminina / Isquemia Miocárdica / Colágeno Tipo IV / Insuficiência Cardíaca Tipo de estudo: Prognostic_studies Limite: Aged / Animals / Humans / Middle aged Idioma: En Ano de publicação: 2016 Tipo de documento: Article