Identification of Asian genotype of chikungunya virus isolated in Mexico.
Virus Genes
; 52(1): 127-9, 2016 Feb.
Article
em En
| MEDLINE
| ID: mdl-26781948
ABSTRACT
We identified 25 autochthonous chikungunya virus cases in Mexico, initially detected by RT-PCR targeting the E1 gene and propagated in C6/36 Aedes albopictus cells, in 2014. To determine the type of virus found, in a previous report, the genomes of 2 CHIKV strains were fully sequenced. Genome sequence analysis revealed that these isolates from Mexico belonged to the Asian genotype, and a phylogenetic association with the circulating strain in the British Virgin Islands was also established in the same year. This was further supported by changes in specific amino acids, E2-V368A and 6K-L20M. For these reasons, it can be inferred that the route of virus entry to Mexico was held across the countries in the Caribbean and Central America. The presence of E1-A226V mutation associated with more efficient replication in the salivary gland of the A. albopictus mosquito was not observed. Interestingly, a newly acquired NSP4-S399C mutation was observed; however, the significance of changes in amino acid found in non-structural proteins in autochthonous strains remains to be elucidated.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus Chikungunya
/
Genoma Viral
/
Febre de Chikungunya
Tipo de estudo:
Diagnostic_studies
País como assunto:
Asia
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Mexico
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article