Do Two Tissue Blood Volume Parameters Measured by Different Near-Infrared Spectroscopy Methods Show the Same Dynamics During Incremental Running?

UNLABELLED: Both the change in total hemoglobin concentration (cHb), assessed by near-infrared continuous-wave spectroscopy (NIR-CWS), and the normalized tissue hemoglobin index (nTHI), assessed by NIR spatially resolved spectroscopy (NIR-SRS), were used to quantify changes in tissue blood volume. However, it is possible that these parameters may show different changes because of the different measurement systems. The present study aimed to compare changes in cHb and nTHI in working muscles, which were selected for measurement because the parameters changed dynamically. METHODS: After a standing rest, seven male runners (age 24±3 years, mean±S.D.) performed an incremental running exercise test on a treadmill (inclination=1%) from 180 to 300 m min(-1). During the tests, cHb and nTHI were monitored from the vastus lateralis (VL) and medial gastrocnemius (GM) muscles. These parameters were relatively evaluated from the minimal to maximal values through the test. RESULTS: When the exercise began, cHb and nTHI quickly decreased and then gradually increased during running. In comparison with both VL and GM, there was significant interaction between cHb and nTHI. CONCLUSIONS: The present results suggest that cHb and nTHI in working muscles are not always synchronized, particularly at the onset of exercise and at high intensities. Although cHb was previously used as the change of tissue blood volume, it is implied that tissue blood volume assessed by cHb is overestimated.