Interaction between the Spliceosomal Pre-mRNA Branch Site and U2 snRNP Protein p14.
Biochemistry
; 55(4): 629-32, 2016 Feb 02.
Article
em En
| MEDLINE
| ID: mdl-26784522
ABSTRACT
We have probed the molecular basis of recognition between human spliceosomal U2 snRNP protein p14 and RNA targets representing the intron branch site region. Interaction of an RNA duplex representing the branch site helix perturbed at least 10 nuclear magnetic resonance cross-peaks of (15)N-labeled p14. However, similar chemical shift changes were observed upon interaction with a duplex without the bulged branch site residue, suggesting that binding of p14 to RNA is nonspecific and does not recognize the branch site. We propose that the p14-RNA interaction screens charges on the backbone of the branch site during spliceosome assembly.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA de Cadeia Dupla
/
Precursores de RNA
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Proteínas de Ligação a RNA
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Spliceossomos
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Ribonucleoproteína Nuclear Pequena U2
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article