Interaction between the Spliceosomal Pre-mRNA Branch Site and U2 snRNP Protein p14.

Perea, William; Schroeder, Kersten T; Bryant, Amy N; Greenbaum, Nancy L

Perea, William; Schroeder, Kersten T; Bryant, Amy N; Greenbaum, Nancy L.

Afiliação

Perea W; Department of Chemistry & Biochemistry, Hunter College of the City University of New York , New York, New York 10065, United States.
Schroeder KT; The Graduate Group in Chemistry, The Graduate Center, City University of New York , New York, New York 10016, United States.
Bryant AN; Department of Chemistry and Biochemistry, Florida State University , Tallahassee, Florida 32306, United States.
Greenbaum NL; Department of Chemistry and Biochemistry, Florida State University , Tallahassee, Florida 32306, United States.

Biochemistry ; 55(4): 629-32, 2016 Feb 02.

Article em En | MEDLINE | ID: mdl-26784522

ABSTRACT

ABSTRACT

We have probed the molecular basis of recognition between human spliceosomal U2 snRNP protein p14 and RNA targets representing the intron branch site region. Interaction of an RNA duplex representing the branch site helix perturbed at least 10 nuclear magnetic resonance cross-peaks of (15)N-labeled p14. However, similar chemical shift changes were observed upon interaction with a duplex without the bulged branch site residue, suggesting that binding of p14 to RNA is nonspecific and does not recognize the branch site. We propose that the p14-RNA interaction screens charges on the backbone of the branch site during spliceosome assembly.

Assuntos

Precursores de RNA/química; RNA de Cadeia Dupla/química; Proteínas de Ligação a RNA/química; Ribonucleoproteína Nuclear Pequena U2/química; Spliceossomos/química; Humanos; Precursores de RNA/genética; Precursores de RNA/metabolismo; RNA de Cadeia Dupla/genética; RNA de Cadeia Dupla/metabolismo; Proteínas de Ligação a RNA/genética; Proteínas de Ligação a RNA/metabolismo; Ribonucleoproteína Nuclear Pequena U2/genética; Ribonucleoproteína Nuclear Pequena U2/metabolismo; Spliceossomos/genética; Spliceossomos/metabolismo

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: RNA de Cadeia Dupla / Precursores de RNA / Proteínas de Ligação a RNA / Spliceossomos / Ribonucleoproteína Nuclear Pequena U2 Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

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