Mycobacterium tuberculosis genotype Beijing: About 15 strains and their part in MDR-TB outbreaks in Algeria.

Within the framework of epidemiological surveillance by molecular typing tools conducted in the NRL on multi-resistant strains, the application of spoligotyping on a group of 390 strains consisting of 389 DR-MTB strains and 1 susceptible strain isolated from patients made it possible to detect the presence of 15 strains belonging to the Beijing genotype. All 15 strains were genotyped by MTBDRplus. Among the 15 strains, 11 were typed by RFLP and 9 by MIRU-VNTR. After analysis of the profiles obtained by the software MIRU-VNTRplus, two spoligotypes (st No. 1 and st No. 265) and four RFLP profiles and a complete identity profile by MIRU-VNTR, information collected on patients allowed the research team to detect a family tie among patients of three different families, as well as one nosocomial TB transmission. The percentage of Beijing strains found among the patients in this study was 3.8%; this figure does not reflect the reality because it was calculated from an essay on MDR-TB. To get an idea of the prevalence of Beijing TB strains in this country, a more extensive study is currently being considered.