Formulation and evaluation of novel glycyrrhizic acid micelles for transdermal delivery of podophyllotoxin.
Drug Deliv
; 23(5): 1623-35, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-26786787
ABSTRACT
CONTEXT As the first-line agent for genital warts, podophyllotoxin (POD) could induce extensively skin burning, itching, and erythema. Meanwhile, as a common anti-inflammatory agent, glycyrrhizic acid (GA), also has amphipathic and solubilizing properties, indicating that it might be a promising drug carrier. OBJECTIVE:
The objective of this study is to formulate and characterize the POD-loaded GA micelles preparation and to evaluate its drug release characteristics and anti-inflammatory properties. MATERIALS ANDMETHODS:
The novel micelles preparation was prepared by ultrasonic dispersion method and characterized using different scanning calorimetries, dynamic light scattering, and transmission electron microscopies. Subsequently, its encapsulation efficiency (EE), drug-loading content (LC), in vitro skin permeation, in vivo drug retention, and distribution of POD were detected. The anti-inflammatory effect of the preparation was reflected by HE staining and immunohistochemistry in the rat skin.RESULTS:
The POD-loaded GA micelles formed spherical shapes (approximately 10 nm) with an EE of 78.53 ± 2.17% and a LC of 7.293 ± 0.42%. Meaningfully, unlike the extensive distribution of the POD tincture throughout the skin tissue, POD released from the POD-loaded GA micelles mainly located in the epidermis and could maintain steady skin retention for 12 h. Moreover, the POD-loaded GA micelles induced less leukocyte infiltration and inflammatory factors (IL-6 and TNF-α) expression when compared with the POD tincture. DISCUSSION ANDCONCLUSION:
Our results suggested that the POD-loaded GA micelles could achieve a higher POD distribution in the epidermal layer as well as a lighter skin inflammation. This new POD delivery system might be a potential and promising candidate for genital warts and deserved further researches.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Podofilotoxina
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Fator de Necrose Tumoral alfa
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Ácido Glicirrízico
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article