Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System.
Methods Mol Biol
; 1350: 95-116, 2016.
Article
em En
| MEDLINE
| ID: mdl-26820855
ABSTRACT
BacMams are modified baculoviruses that contain mammalian expression cassettes for gene delivery and expression in mammalian cells. BacMams have become an integral part of the recombinant mammalian gene expression toolbox in research labs worldwide. Construction of transfer vectors is straightforward using basic molecular biology protocols. Virus generation is based on common methods used with the baculovirus insect cell expression system. BacMam transduction of mammalian cells requires minimal modifications to familiar cell culture methods. This chapter highlights the BacMam transfer vector pHTBV.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
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Baculoviridae
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article