Cellular mRNA recruits the ribosome via eIF3-PABP bridge to initiate internal translation.
RNA Biol
; 14(5): 553-567, 2017 05 04.
Article
em En
| MEDLINE
| ID: mdl-26828225
ABSTRACT
IRES-mediated translation of key cell fate regulating genes has been implicated in tumorigenesis. Concerted action of canonical eukaryotic initiation factors and IRES transacting factors (ITAFs) was shown to regulate cellular IRES mediated translation; however, the precise molecular mechanism of ribosome recruitment to cellular IRESes remains unclear. Here we show that the X-linked inhibitor of apoptosis (XIAP) IRES operates in an evolutionary conserved viral like mode and the structural integrity, particularly in the vicinity of AUG, is critical for ribosome recruitment. The binding of eIF3 together with PABP potentiates ribosome recruitment to the IRES. Our data support the model in which eIF3 binds directly to the XIAP IRES RNA in a structure-dependent manner and acts as a scaffold for IRES RNA, PABP and the 40S ribosome.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribossomos
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Biossíntese de Proteínas
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RNA Mensageiro
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Proteínas de Ligação a Poli(A)
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Fator de Iniciação 3 em Eucariotos
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Proteínas Inibidoras de Apoptose Ligadas ao Cromossomo X
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Sítios Internos de Entrada Ribossomal
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article