Nanoparticle-mediated photothermal effect enables a new method for quantitative biochemical analysis using a thermometer.
Nanoscale
; 8(10): 5422-7, 2016 Mar 14.
Article
em En
| MEDLINE
| ID: mdl-26838516
ABSTRACT
A new biomolecular quantitation method, nanoparticle-mediated photothermal bioassay, using a common thermometer as the signal reader was developed. Using an immunoassay as a proof of concept, iron oxide nanoparticles (NPs) captured in the sandwich-type assay system were transformed into a near-infrared (NIR) laser-driven photothermal agent, Prussian blue (PB) NPs, which acted as a photothermal probe to convert the assay signal into heat through the photothermal effect, thus allowing sensitive biomolecular quantitation using a thermometer. This is the first report of biomolecular quantitation using a thermometer and also serves as the first attempt to introduce the nanoparticle-mediated photothermal effect for bioassays.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos Férricos
/
Nanotecnologia
/
Nanopartículas Metálicas
Limite:
Humans
/
Male
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article