Accurate and sensitive total genomic DNA methylation analysis from sub-nanogram input with embedded SERS nanotags.
Chem Commun (Camb)
; 52(17): 3560-3, 2016 Feb 28.
Article
em En
| MEDLINE
| ID: mdl-26841713
ABSTRACT
Sensitive and accurate total genomic DNA methylation analysis was demonstrated by surface-enhanced Raman scattering (SERS) via embedded internal SERS nanotags. The assay was sensitive to 0.2 ng input DNA while differentiating as low as 6.25% changes in DNA methylation. The method could also successfully differentiate cells before and after de-methylating drug-treatment and between tumor and normal biopsies.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Metilação de DNA
/
Genômica
/
Nanotecnologia
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article