Mapping immune cell infiltration using restricted diffusion MRI.
Magn Reson Med
; 77(2): 603-612, 2017 02.
Article
em En
| MEDLINE
| ID: mdl-26843524
ABSTRACT
PURPOSE:
Diffusion MRI provides a noninvasive way to assess tissue microstructure. Based on diffusion MRI, we propose a model-free method called restricted diffusion imaging (RDI) to quantify restricted diffusion and correlate it with cellularity. THEORY ANDMETHODS:
An analytical relation between q-space signals and the density of restricted spins was derived to quantify restricted diffusion. A phantom study was conducted to investigate the performance of RDI, and RDI was applied to an animal study to assess immune cell infiltration in myocardial tissues with ischemia-reperfusion injury.RESULTS:
Our phantom study showed a correlation coefficient of 0.998 between cell density and the restricted diffusion quantified by RDI. The animal study also showed that the high-value regions in RDI matched well with the macrophage infiltration areas in the H&E stained slides. In comparison with diffusion tensor imaging (DTI), RDI exhibited its outperformance to detect macrophage infiltration and delineate inflammatory myocardium.CONCLUSION:
RDI can be used to reveal cell density and detect immune cell infiltration. RDI exhibits better specificity than the diffusivity measurement derived from DTI. Magn Reson Med 77603-612, 2017. © 2016 International Society for Magnetic Resonance in Medicine.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Interpretação de Imagem Assistida por Computador
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Traumatismo por Reperfusão Miocárdica
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Infiltração de Neutrófilos
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Imagem de Difusão por Ressonância Magnética
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Rastreamento de Células
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Macrófagos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article