ß-Catenin Expression in Oropharyngeal Squamous Cell Carcinomas: Comparison and Correlation with p16 and Human Papillomavirus in situ Hybridization.
Acta Cytol
; 59(6): 479-84, 2015.
Article
em En
| MEDLINE
| ID: mdl-26849661
ABSTRACT
BACKGROUND:
The Wnt/ß-catenin signaling pathway has been noted to be upregulated in head and neck cancers, including oropharyngeal squamous cell carcinoma (OSCC). This study compared the efficacy of ß-catenin immunohistochemistry (IHC), p16 IHC and automated human papillomavirus (HPV) in situ hybridization (ISH) in OSCC.METHODS:
Sixty-eight OSCCs (48 surgical specimens and 20 fine-needle aspirations) were evaluated. Nuclear staining only of ß-catenin was assessed as 0-3+ intensity (relative to controls of benign squamous mucosa). p16 was interpreted as positive if 70% of tumor cells showed brown nuclear and cytoplasmic staining. HPV ISH was interpreted as positive if a minimum of one tumor cell showed brown punctate dot-like nuclear positivity. p16 IHC and HPV ISH were then correlated with ß-catenin staining. HPV ISH was used as the gold standard.RESULTS:
Twenty-five of 48 surgical specimens (52.1%) and 11 of 20 cell blocks (55%) stained positively for ß-catenin, making a total of 36 of 68 (52.9%) staining positively for ß-catenin, as compared to 61.7% positive for p16 IHC and 70.6% positive by automated HPV ISH, the gold standard method for OSCC diagnosis. x03C7;2 analysis revealed no significant correlation between ß-catenin and HPV ISH (p > 0.05) and demonstrated a strong correlation between p16 and HPV ISH (p < 0.05).CONCLUSION:
ß-Catenin IHC is not a sensitive or specific marker of HPV and is unlikely to be a useful adjunct to p16 IHC or HPV ISH in the setting of advanced OSCC. However, as this study focused on samples of advanced OSCC, ß-catenin IHC may still find some use in the diagnosis of early-stage OSCC.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
DNA Viral
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Imuno-Histoquímica
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Carcinoma de Células Escamosas
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Neoplasias Orofaríngeas
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Hibridização In Situ
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Inibidor p16 de Quinase Dependente de Ciclina
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Infecções por Papillomavirus
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Beta Catenina
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Testes de DNA para Papilomavírus Humano
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2015
Tipo de documento:
Article