LINE-1 Cultured Cell Retrotransposition Assay.
Methods Mol Biol
; 1400: 139-56, 2016.
Article
em En
| MEDLINE
| ID: mdl-26895052
ABSTRACT
The Long INterspersed Element-1 (LINE-1 or L1) retrotransposition assay has facilitated the discovery and characterization of active (i.e., retrotransposition-competent) LINE-1 sequences from mammalian genomes. In this assay, an engineered LINE-1 containing a retrotransposition reporter cassette is transiently transfected into a cultured cell line. Expression of the reporter cassette, which occurs only after a successful round of retrotransposition, allows the detection and quantification of the LINE-1 retrotransposition efficiency. This assay has yielded insight into the mechanism of LINE-1 retrotransposition. It also has provided a greater understanding of how the cell regulates LINE-1 retrotransposition and how LINE-1 retrotransposition impacts the structure of mammalian genomes. Below, we provide a brief introduction to LINE-1 biology and then detail how the LINE-1 retrotransposition assay is performed in cultured mammalian cells.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Elementos Nucleotídeos Longos e Dispersos
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article