Yeast repressor alpha 2 binds to its operator cooperatively with yeast protein Mcm1.
Mol Cell Biol
; 9(11): 5228-30, 1989 Nov.
Article
em En
| MEDLINE
| ID: mdl-2689875
ABSTRACT
To bring about repression of a family fo genes in Saccharomyces cerevisiae called the a-specific genes, two transcriptional regulatory proteins, alpha 2 and GRM (general regulator of matin type), bind cooperatively to an operator found upstream of each a-specific gene. To date, GRM has been defined only biochemically. In this communication we show that the product of a single yeast gene (MCM1) is sufficient to bind cooperatively with alpha 2 to the operator. We also show that antiserum raised against the MCM1 gene product recognizes GRM from yeast cells. These results, in combination with previous observations, provide strong evidence that MCM1 encodes the GRM activity.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
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Saccharomyces cerevisiae
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Fatores de Transcrição
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Proteínas Fúngicas
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Regiões Operadoras Genéticas
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Genes Fúngicos
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article