Cell-free ribosome display and selection of antibodies on arrayed antigens.
Proteomics
; 16(8): 1291-6, 2016 Apr.
Article
em En
| MEDLINE
| ID: mdl-26899874
ABSTRACT
In vitro display technology is a powerful tool for discovery and optimisation of novel antibodies. With increasing demands on various binding molecules in proteomics studies, techniques for a large-scale generation of antibodies or antibody fragments are needed. Here, we describe a novel method for parallel generation of different antibody fragments (scFv) by integrating cell-free ribosome display with array technology. We have demonstrated the procedure by successfully isolating scFv antibodies specific to 16 different cancer biomarkers via a single process. Our results provide proof of principle for multiple production of various scFv antibodies simultaneously.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ribossomos
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Análise Serial de Proteínas
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Anticorpos de Cadeia Única
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Antígenos
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Neoplasias
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article