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Comparing the ability of luminex xMAP(®) salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern Chinese population.
Liang, D W; Lu, J H; Wu, Q; Ke, B X; Jiang, C H; Long, J; Fang, Y P; Lin, L J; Zeng, N Y; Fu, L; Jiang, L X.
Afiliação
  • Liang DW; Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • Lu JH; Department of Laboratory Medicine, The First People's Hospital of Shunde District, Foshan, China.
  • Wu Q; Haizhu district Center for Disease Control and Prevention, Guangzhou, China.
  • Ke BX; Institute of Microbiology, Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China.
  • Jiang CH; Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • Long J; Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • Fang YP; Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • Lin LJ; Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • Zeng NY; Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • Fu L; Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • Jiang LX; Center of Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
J Appl Microbiol ; 120(6): 1668-76, 2016 Jun.
Article em En | MEDLINE | ID: mdl-26914944
AIMS: To investigate whether the Luminex xMAP(®) Salmonella Serotyping Assay (xMAP SSA) is applicable to serotype Salmonella from humans in southern China. METHODS AND RESULTS: Two hundred and five Salmonella isolates from diarrhoea patients were serotyped by xMAP SSA in parallel with the traditional serotyping. Forty serotypes were identified among 205 isolates; the most prevalent serotypes identified were Salmonella Enteritidis, Salmonella Stanley, Salmonella I 4,5,12:i:-, and Salmonella Typhimurium. One hundred and ninety-five (95·1%, 195/205) isolates were serotyped completely by xMAP SSA, while 10 stereotypes were partially detected as they were not included in the assay. The xMAP SSA correctly identified 192 (98·5%, 192/195) isolates. Five nonmotile and three monophasic strains, which possessed flagellar antigen genes that weren't expressed, were completely serotyped by xMAP SSA; however, these isolates were left undetected by the traditional method. CONCLUSION: The xMAP SSA used in the study is a microsphere-based, molecular assay that could rapidly and accurately serotype Salmonella. It is suitable to identify the serovars of Salmonella in southern China. SIGNIFICANCE AND IMPACT OF THE STUDY: The xMAP SSA, with high-throughput characteristics, provides an accurate and rapid serotyping system that dramatically strengthens the capability of clinical and public health laboratories for Salmonella serotyping.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Salmonella / Infecções por Salmonella / Sorotipagem Tipo de estudo: Prognostic_studies Limite: Humans País como assunto: Asia Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Salmonella / Infecções por Salmonella / Sorotipagem Tipo de estudo: Prognostic_studies Limite: Humans País como assunto: Asia Idioma: En Ano de publicação: 2016 Tipo de documento: Article