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Bioproduction of D-Tagatose from D-Galactose Using Phosphoglucose Isomerase from Pseudomonas aeruginosa PAO1.
Patel, Manisha J; Patel, Arti T; Akhani, Rekha; Dedania, Samir; Patel, Darshan H.
Afiliação
  • Patel MJ; P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, Changa, 388 421, Gujarat, India.
  • Patel AT; P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, Changa, 388 421, Gujarat, India.
  • Akhani R; P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, Changa, 388 421, Gujarat, India.
  • Dedania S; P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, Changa, 388 421, Gujarat, India.
  • Patel DH; P. D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, Changa, 388 421, Gujarat, India. darshanpatel.bt@charusat.ac.in.
Appl Biochem Biotechnol ; 179(5): 715-27, 2016 Jul.
Article em En | MEDLINE | ID: mdl-26922727
Pseudomonas aeruginosa PAO1 phosphoglucose isomerase was purified as an active soluble form by a single-step purification using Ni-NTA chromatography that showed homogeneity on SDS-PAGE with molecular mass ∼62 kDa. The optimum temperature and pH for the maximum isomerization activity with D-galactose were 60 °C and 7.0, respectively. Generally, sugar phosphate isomerases show metal-independent activity but PA-PGI exhibited metal-dependent isomerization activity with aldosugars and optimally catalyzed the D-galactose isomerization in the presence of 1.0 mM MnCl2. The apparent Km and Vmax for D-galactose under standardized conditions were calculated to be 1029 mM (±31.30 with S.E.) and 5.95 U/mg (±0.9 with S.E.), respectively. Equilibrium reached after 180 min with production of 567.51 µM D-tagatose from 1000 mM of D-galactose. Though, the bioconversion ratio is low but it can be increased by immobilization and enzyme engineering. Although various L-arabinose isomerases have been characterized for bioproduction of D-tagatose, P. aeruginosa glucose phosphate isomerase is distinguished from the other L-arabinose isomerases by its optimal temperature (60 °C) for D-tagatose production being mesophilic bacteria, making it an alternate choice for bulk production.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Glucose-6-Fosfato Isomerase / Hexoses Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pseudomonas aeruginosa / Glucose-6-Fosfato Isomerase / Hexoses Idioma: En Ano de publicação: 2016 Tipo de documento: Article