Studying the regulation of endosomal cAMP production in GPCR signaling.
Methods Cell Biol
; 132: 109-26, 2016.
Article
em En
| MEDLINE
| ID: mdl-26928541
ABSTRACT
We describe methods based on live cell fluorescent microscopy and mass spectrometry to characterize the mechanism of endosomal cAMP production and its regulation using the parathyroid hormone (PTH) type 1 receptor as a prime example. These methods permit to measure rapid changes of cAMP levels in response to PTH, kinetics of endosomal ligand-receptor interaction, pH changes associated with receptor trafficking, and to identify the endosomal receptor interactome.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sistemas do Segundo Mensageiro
/
AMP Cíclico
/
Receptor Tipo 1 de Hormônio Paratireóideo
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article