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Studying the regulation of endosomal cAMP production in GPCR signaling.
Gidon, Alexandre; Feinstein, Timothy N; Xiao, Kunhong; Vilardaga, Jean-Pierre.
Afiliação
  • Gidon A; Molecular Mechanisms of Mycobacterial Infection, Center for Molecular Inflammation Research, Norwegian University of Science and Technology, Trondheim, Norway.
  • Feinstein TN; Department of Developmental Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
  • Xiao K; Laboratory for GPCR Biology, Department of Pharmacology & Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
  • Vilardaga JP; Laboratory for GPCR Biology, Department of Pharmacology & Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Methods Cell Biol ; 132: 109-26, 2016.
Article em En | MEDLINE | ID: mdl-26928541
ABSTRACT
We describe methods based on live cell fluorescent microscopy and mass spectrometry to characterize the mechanism of endosomal cAMP production and its regulation using the parathyroid hormone (PTH) type 1 receptor as a prime example. These methods permit to measure rapid changes of cAMP levels in response to PTH, kinetics of endosomal ligand-receptor interaction, pH changes associated with receptor trafficking, and to identify the endosomal receptor interactome.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas do Segundo Mensageiro / AMP Cíclico / Receptor Tipo 1 de Hormônio Paratireóideo Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sistemas do Segundo Mensageiro / AMP Cíclico / Receptor Tipo 1 de Hormônio Paratireóideo Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article