Isolation and whole genome sequencing of a Ruminococcus-like bacterium, associated with irritable bowel syndrome.
Anaerobe
; 39: 60-7, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-26946362
ABSTRACT
In our previous studies on the intestinal microbiota in irritable bowel syndrome (IBS), we identified a bacterial phylotype with higher abundance in patients suffering from diarrhea than in healthy controls. In the present work, we have isolated in pure culture strain RT94, belonging to this phylotype, determined its whole genome sequence and performed an extensive genomic analysis and phenotypical testing. This revealed strain RT94 to be a strict anaerobe apparently belonging to a novel species with only 94% similarity in the 16S rRNA gene sequence to the closest relatives Ruminococcus torques and Ruminococcus lactaris. The G + C content of strain RT94 is 45.2 mol% and the major long-chain cellular fatty acids are C160, C180 and C140. The isolate is metabolically versatile but not a mucus or cellulose utilizer. It produces acetate, ethanol, succinate, lactate and formate, but very little butyrate, as end products of glucose metabolism. The mechanisms underlying the association of strain RT94 with diarrhea-type IBS are discussed.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bactérias Anaeróbias
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RNA Ribossômico 16S
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Infecções por Bactérias Gram-Positivas
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Genoma Bacteriano
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Ruminococcus
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Síndrome do Intestino Irritável
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Diarreia
Tipo de estudo:
Prognostic_studies
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Risk_factors_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article