Peripheral blood collection: the first step towards gene expression profiling.
Biomarkers
; 21(5): 458-65, 2016 Jul.
Article
em En
| MEDLINE
| ID: mdl-26984061
ABSTRACT
A crucial challenge for gene expression analysis in human biomonitoring studies on whole blood samples is rapid sample handling and mRNA stabilization. This study was designed to evaluate the impact of short bench times (less than 30 min) on yield, quality and gene expression of mRNA in the presence of different stabilization buffers (Tempus(TM) Blood RNA tube and RNAlater(®) Stabilization Reagent). Microarray analyzes showed significant changes over short periods of time in expression of a considerate part of the transcriptome (2356 genes) with a prominent role for NFкB-, cancer- and glucocorticoid-mediated networks, and specifically interleukin-8 (IL-8). These findings suggest that even short bench times affect gene expression, requiring to carry out blood collection in a strictly standardized way.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Coleta de Amostras Sanguíneas
/
Perfilação da Expressão Gênica
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article