Risungbinella massiliensis sp. nov., a new member of Thermoactinomycetaceae isolated from human gut.
Antonie Van Leeuwenhoek
; 109(6): 773-84, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-26984352
ABSTRACT
A novel filamentous bacterium, designated GD1(T), was isolated from the gut microbiota of a 38-year-old male who suffered from a Coxiella burnetii vascular for which he received multiple a broad-spectrum antibiotic cocktail at the time of the stool collection. The strain was isolated as a part of culturomics study by cultivation on 5 % sheep blood agar in aerobic condition at 28 °C, after 14 days of incubation. Strain GD1(T) shows 16S rRNA gene sequence similarities of 98.01 % to the type strain of Risungbinella pyongyangensis. We describe here the features of this bacterium, together with the complete genome sequence and annotation. The G+C content of the genomic DNA was determined to be 40.1 mol %. The major fatty acids of strain GD1(T) were identified as iso-C150, iso-C170, anteiso-C150, iso-C140 and C160. The 3,440,191 bp long genome contains 3540 protein-coding and 67 RNA genes, including three rRNA genes. Strain GD1(T) (= DSM 46691 = CSUR P1082) sp. nov. is here classified as the type strain of a new species, Risungbinella massiliensis, within the family Thermoactinomycetaceae. To date, strain GD1(T) is the first member of the family Thermoactinomycetaceae isolated from human gut and the fourth from a human specimen.
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Base de dados:
MEDLINE
Assunto principal:
Bacillales
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Intestinos
Limite:
Adult
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Humans
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Male
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article