Effect of Utipro(®) (containing gelatin-xyloglucan) against Escherichia coli invasion of intestinal epithelial cells: results of an in vitro study.
Future Microbiol
; 11: 651-8, 2016 05.
Article
em En
| MEDLINE
| ID: mdl-27022857
ABSTRACT
AIM:
To evaluate whether Utipro(®), a natural product approved to prevent urinary tract infections, protects intestinal epithelial cells from Escherichia coli adherence/intracellular invasion in vitro. MATERIALS &METHODS:
Caco-2 and CacoGoblet(TM) cells were treated with Utipro (1.5 to 10 mg/ml) or untreated (controls). E. coli adherence/intracellular invasion was evaluated by Trans-Epithelial Electrical Resistance, Lucifer Yellow assay and microbial counts.RESULTS:
Utipro was noncytotoxic. Utipro 5 and 10 mg/ml protected cell tight junctions (mean ± SD Trans-Epithelial Electrical Resistance [Ω × cm(2)] 66.83 ± 0.29 and 71.33 ± 0.29, respectively), and protected cells from E. coli intracellular invasion (mean ± SD reductions in total bacteria counts [Log10] 0.9 ± 0.06 and 2.1 ± 0.56, respectively).CONCLUSION:
Results indicate that Utipro creates a protective physical barrier on intestinal epithelial cells in vitro which reduces the settling of E. coli reservoirs. These results constitute the first step in the demonstration of the efficacy of Utipro to prevent urinary tract infections. Further research is needed in in vivo models and clinical trials.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Xilanos
/
Aderência Bacteriana
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Células Epiteliais
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Escherichia coli
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Infecções por Escherichia coli
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Gelatina
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Glucanos
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Intestinos
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article