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Antimicrobial peptide (Cn-AMP2) from liquid endosperm of Cocos nucifera forms amyloid-like fibrillar structure.
Gour, Shalini; Kaushik, Vibha; Kumar, Vijay; Bhat, Priyanka; Yadav, Subhash C; Yadav, Jay K.
Afiliação
  • Gour S; Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
  • Kaushik V; Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
  • Kumar V; Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
  • Bhat P; Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
  • Yadav SC; Department of Anatomy, All India Institute of Medical Sciences, New Delhi, -110029, India.
  • Yadav JK; Department of Biotechnology, Central University of Rajasthan, Bandersindri NH-8 Jaipur-Ajmer Highway, Dist., Ajmer, 305 801, Rajasthan, India.
J Pept Sci ; 22(4): 201-7, 2016 Apr.
Article em En | MEDLINE | ID: mdl-27028204
ABSTRACT
Cn-AMP2 is an antimicrobial peptide derived from liquid endosperm of coconut (Cocos nucifera). It consists of 11 amino acid residues and predicted to have high propensity for ß-sheet formation that disposes this peptide to be amyloidogenic. In the present study, we have examined the amyloidogenic propensities of Cn-AMP2 in silico and then tested the predictions under in vitro conditions. The in silico study revealed that the peptide possesses high amyloidogenic propensity comparable with Aß. Upon solubilisation and agitation in aqueous buffer, Cn-AMP2 forms visible aggregates that display bathochromic shift in the Congo red absorbance spectra, strong increase in thioflavin T fluorescence and fibrillar morphology under transmission electron microscopy. All these properties are typical of an amyloid fibril derived from various proteins/peptides including Aß.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Cocos / Peptídeos Catiônicos Antimicrobianos / Endosperma Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Cocos / Peptídeos Catiônicos Antimicrobianos / Endosperma Idioma: En Ano de publicação: 2016 Tipo de documento: Article