Correlation of Histologic Features with In Vivo Imaging of Reticular Pseudodrusen.
Ophthalmology
; 123(6): 1320-31, 2016 06.
Article
em En
| MEDLINE
| ID: mdl-27039021
ABSTRACT
PURPOSE:
To determine the histologic and cellular correlates in the retina and retinal pigment epithelium (RPE) with the presence of optical coherence tomography-defined reticular pseudodrusen (RPD).DESIGN:
Observation case using immunocytochemistry of an exenterated eye with immediate fixation after removal.PARTICIPANTS:
Two patients, one with confirmed RPD and the other with mid-peripheral drusen, underwent multimethod imaging before exenteration and immediate fixation of the posterior eyecup for high-resolution immunocytochemical analysis.METHODS:
Optical coherence tomography (OCT) was compared with high-resolution immunocytochemistry using a range of cellular markers to determine changes in the RPE, photoreceptors, and gliosis. MAIN OUTCOMEMEASURES:
Correlations of the appearance of reticular pseudodrusen on OCT and immunocytochemical analysis.RESULTS:
Reticular pseudodrusen were deposits juxtaposed to photoreceptor outer segments extending through the outer nuclear layer and even beyond the outer limiting membrane. Deposits were rich in vitronectin, photoreceptor-associated proteins, and Iba1-immunoreactive immune cells. In contrast to conventional drusen the lipid stain Oil Red O failed to stain RPD. Cellular analysis revealed that RPD were associated with photoreceptor disruption and loss and localized gliosis. In addition, anomalies in the RPE were observed.CONCLUSIONS:
Reticular pseudodrusen represent subretinal deposits that extend through the outer nuclear layer, affect photoreceptor integrity, and are associated with retinal gliosis and RPE damage.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retina
/
Drusas Retinianas
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Tomografia de Coerência Óptica
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Epitélio Pigmentado da Retina
Limite:
Aged80
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article