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Generation and characterization of ß1,2-gluco-oligosaccharide probes from Brucella abortus cyclic ß-glucan and their recognition by C-type lectins of the immune system.
Zhang, Hongtao; Palma, Angelina S; Zhang, Yibing; Childs, Robert A; Liu, Yan; Mitchell, Daniel A; Guidolin, Leticia S; Weigel, Wilfried; Mulloy, Barbara; Ciocchini, Andrés E; Feizi, Ten; Chai, Wengang.
Afiliação
  • Zhang H; Glycosciences Laboratory, Department of Medicine, Imperial College London, London W12 0NN, UK.
  • Palma AS; Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
  • Zhang Y; Glycosciences Laboratory, Department of Medicine, Imperial College London, London W12 0NN, UK angelina.palma@fct.unl.pt w.chai@imperial.ac.uk.
  • Childs RA; UCIBIO-REQUIMTE, Department of Chemistry, Faculty of Science and Technology, NOVA Universidade de Lisboa, Caparica 2829-516, Portugal.
  • Liu Y; Glycosciences Laboratory, Department of Medicine, Imperial College London, London W12 0NN, UK.
  • Mitchell DA; Glycosciences Laboratory, Department of Medicine, Imperial College London, London W12 0NN, UK.
  • Guidolin LS; Glycosciences Laboratory, Department of Medicine, Imperial College London, London W12 0NN, UK.
  • Weigel W; CSRI-UHCW, Walsgrave Campus, University of Warwick, Coventry CV2 2DX, UK.
  • Mulloy B; Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo A. Ugalde", Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín, San Martín, Buenos Aires 1650, Argentina.
  • Ciocchini AE; SCIENION AG, Volmerstrasse 7b, Berlin 12489, Germany.
  • Feizi T; Glycosciences Laboratory, Department of Medicine, Imperial College London, London W12 0NN, UK.
  • Chai W; Instituto de Investigaciones Biotecnológicas "Dr. Rodolfo A. Ugalde", Instituto Tecnológico de Chascomús (IIB-INTECH), Universidad Nacional de San Martín, San Martín, Buenos Aires 1650, Argentina.
Glycobiology ; 26(10): 1086-1096, 2016 10.
Article em En | MEDLINE | ID: mdl-27053576
The ß1,2-glucans produced by bacteria are important in invasion, survival and immunomodulation in infected hosts be they mammals or plants. However, there has been a lack of information on proteins which recognize these molecules. This is partly due to the extremely limited availability of the sequence-defined oligosaccharides and derived probes for use in the study of their interactions. Here we have used the cyclic ß1,2-glucan (CßG) of the bacterial pathogen Brucella abortus, after removal of succinyl side chains, to prepare linearized oligosaccharides which were used to generate microarrays. We describe optimized conditions for partial depolymerization of the cyclic glucan by acid hydrolysis and conversion of the ß1,2-gluco-oligosaccharides, with degrees of polymerization 2-13, to neoglycolipids for the purpose of generating microarrays. By microarray analyses, we show that the C-type lectin receptor DC-SIGNR, like the closely related DC-SIGN we investigated earlier, binds to the ß1,2-gluco-oligosaccharides, as does the soluble immune effector serum mannose-binding protein. Exploratory studies with DC-SIGN are suggestive of the recognition also of the intact CßG by this receptor. These findings open the way to unravelling mechanisms of immunomodulation mediated by ß1,2-glucans in mammalian systems.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Brucella abortus / Sondas Moleculares / Lectinas Tipo C Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Brucella abortus / Sondas Moleculares / Lectinas Tipo C Idioma: En Ano de publicação: 2016 Tipo de documento: Article