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Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol.
van der Woude, Aniek D; Perez Gallego, Ruth; Vreugdenhil, Angie; Puthan Veetil, Vinod; Chroumpi, Tania; Hellingwerf, Klaas J.
Afiliação
  • van der Woude AD; Photanol BV, Science Park 408, 1098XH, Amsterdam, The Netherlands. aniek.vanderwoude@photanol.com.
  • Perez Gallego R; Photanol BV, Science Park 408, 1098XH, Amsterdam, The Netherlands.
  • Vreugdenhil A; Photanol BV, Science Park 408, 1098XH, Amsterdam, The Netherlands.
  • Puthan Veetil V; Photanol BV, Science Park 408, 1098XH, Amsterdam, The Netherlands.
  • Chroumpi T; Photanol BV, Science Park 408, 1098XH, Amsterdam, The Netherlands.
  • Hellingwerf KJ; Photanol BV, Science Park 408, 1098XH, Amsterdam, The Netherlands.
Microb Cell Fact ; 15: 60, 2016 Apr 08.
Article em En | MEDLINE | ID: mdl-27059824
BACKGROUND: Erythritol is a polyol that is used in the food and beverage industry. Due to its non-caloric and non-cariogenic properties, the popularity of this sweetener is increasing. Large scale production of erythritol is currently based on conversion of glucose by selected fungi. In this study, we describe a biotechnological process to produce erythritol from light and CO2, using engineered Synechocystis sp. PCC6803. METHODS: By functionally expressing codon-optimized genes encoding the erythrose-4-phosphate phosphatase TM1254 and the erythrose reductase Gcy1p, or GLD1, this cyanobacterium can directly convert the Calvin cycle intermediate erythrose-4-phosphate into erythritol via a two-step process and release the polyol sugar in the extracellular medium. Further modifications targeted enzyme expression and pathway intermediates. CONCLUSIONS: After several optimization steps, the best strain, SEP024, produced up to 2.1 mM (256 mg/l) erythritol, excreted in the medium.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Engenharia Genética / Synechocystis / Eritritol Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Engenharia Genética / Synechocystis / Eritritol Idioma: En Ano de publicação: 2016 Tipo de documento: Article