Endothelial, platelet, and macrophage microparticle levels do not change acutely following transcatheter aortic valve replacement.
J Negat Results Biomed
; 15: 7, 2016 Apr 11.
Article
em En
| MEDLINE
| ID: mdl-27063005
ABSTRACT
BACKGROUND:
Patients with severe aortic stenosis have increased levels of prothrombotic and proinflammatory microparticles (MP), and MPs actively regulate pathological processes that lead to atherothrombotic cardiovascular events. Shear stress is a validated stimulus of MP production, and abnormal shear stress in aortic stenosis increases MP release in ex-vivo studies. We hypothesized that in patients with severe aortic stenosis, percutaneous replacement of the aortic valve (TAVR) would reduce abnormal shear stress and would decrease levels of circulating MPs.FINDINGS:
The experimental protocol utilized flow cytometry (FC) and nanoparticle tracking analysis (NTA) to quantify circulating plasma MP levels in aortic stenosis patients at baseline and 5 days after TAVR. The baseline and 5 day MP counts measured by FC were 6.10â 10(5) ± 1.21â 10(5) MP/µL and 5.74â 10(5) ± 9.54â 10(4) MP/µL, respectively (p = 0.91). The baseline and 5 day MP counts measured by NTA were 9.29â 10(13) ± 1.66â 10(13) MP/µL and 3.95â 10(14) ± 3.11â 10(14) MP/µL, respectively (p = 0.91). When MPs were stratified by cell source, there was no difference in pre/post TAVR endothelial, platelet, or leukocyte MP levels.CONCLUSION:
Levels of circulating MPs do not change acutely following TAVR therapy for aortic stenosis. Trial registered at clinicaltrials.gov NCT02193035 on July 11, 2014.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Valva Aórtica
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Plaquetas
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Próteses Valvulares Cardíacas
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Endotélio Vascular
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Substituição da Valva Aórtica Transcateter
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Macrófagos
Tipo de estudo:
Guideline
Limite:
Aged
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Aged80
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Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article