Enhancer activity of Helitron in sericin-1 gene promoter from Bombyx mori.
Insect Sci
; 23(3): 396-405, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-27067405
ABSTRACT
Sericin is a kind of water-soluble protein expressed specifically in the middle silk gland of Bombyx mori. When the sericin-1 gene promoter was cloned and a transgenic vector was constructed to express a foreign protein, a specific Helitron, Bmhel-8, was identified in the sericin-1 gene promoter sequence in some genotypes of Bombyx mori and Bombyx mandarina. Given that the Bmhel-8 Helitron transposon was present only in some genotypes, it could be the source of allelic variation in the sericin-1 promoter. The length of the sericin-1 promoter sequence is approximately 1063 or 643 bp. The larger size of the sequence or allele is ascribed to the presence of Bmhel-8. Silkworm genotypes can be homozygous for either the shorter or larger promoter sequence or heterozygous, containing both alleles. Bmhel-8 in the sericin-1 promoter exhibits enhancer activity, as demonstrated by a dual-luciferase reporter system in BmE cell lines. Furthermore, Bmhel-8 displays enhancer activity in a sericin-1 promoter-driven gene expression system but does not regulate the tissue-specific expression of sericin-1.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bombyx
/
Elementos de DNA Transponíveis
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Sericinas
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article