Postnatal development of the dopaminergic signaling involved in the modulation of intestinal motility in mice.
Pediatr Res
; 80(3): 440-7, 2016 09.
Article
em En
| MEDLINE
| ID: mdl-27089499
ABSTRACT
BACKGROUND:
Since antidopaminergic drugs are pharmacological agents employed in the management of gastrointestinal motor disorders at all ages, we investigated whether the enteric dopaminergic system may undergo developmental changes after birth.METHODS:
Intestinal mechanical activity was examined in vitro as changes in isometric tension.RESULTS:
In 2-d-old (P2) mice, dopamine induced a contractile effect, decreasing in intensity with age, replaced, at the weaning (day 20), by a relaxant response. Both responses were tetrodotoxin (TTX)-insensitive. In P2, dopaminergic contraction was inhibited by D1-like receptor antagonist and mimicked by D1-like receptor agonist. In 90-d-old (P90) mice, the relaxation was reduced by both D1- and D2-like receptor antagonists, and mimicked by D1- and D2-like receptor agonists. In P2, contraction was antagonized by phospholipase C inhibitor, while in P90 relaxation was antagonized by adenylyl cyclase inhibitor and potentiated by phospholipase C inhibitor. The presence of dopamine receptors was assessed by immunofluorescence. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed a significant increase in D1, D2, and D3 receptor expression in proximal intestine with the age.CONCLUSION:
In mouse small intestine, the response to dopamine undergoes developmental changes shifting from contraction to relaxation at weaning, as the consequence of D2-like receptor recruitment and increased expression of D1 receptors.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Dopamina
/
Motilidade Gastrointestinal
/
Intestino Delgado
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article