Genome-wide identification of Wnt/ß-catenin transcriptional targets during Xenopus gastrulation.
Dev Biol
; 426(2): 165-175, 2017 06 15.
Article
em En
| MEDLINE
| ID: mdl-27091726
ABSTRACT
The canonical Wnt/ß-catenin signaling pathway plays multiple roles during Xenopus gastrulation, including posteriorization of the neural plate, patterning of the mesoderm, and induction of the neural crest. Wnt signaling stabilizes ß-catenin, which then activates target genes. However, few targets of this signaling pathway that mediate early developmental processes are known. Here we sought to identify transcriptional targets of the Wnt/ß-catenin signaling pathway using a genome-wide approach. We selected putative targets using the criteria of reduced expression upon zygotic Wnt knockdown, ß-catenin binding within 50kb of the gene, and expression in tissues that receive Wnt signaling. Using these criteria, we found 21 novel direct transcriptional targets of Wnt/ß-catenin signaling during gastrulation and in addition have identified putative regulatory elements for further characterization in future studies.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Xenopus laevis
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Gastrulação
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Via de Sinalização Wnt
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Gástrula
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article