Light-driven dinitrogen reduction catalyzed by a CdS:nitrogenase MoFe protein biohybrid.
Science
; 352(6284): 448-50, 2016 Apr 22.
Article
em En
| MEDLINE
| ID: mdl-27102481
ABSTRACT
The splitting of dinitrogen (N2) and reduction to ammonia (NH3) is a kinetically complex and energetically challenging multistep reaction. In the Haber-Bosch process, N2 reduction is accomplished at high temperature and pressure, whereas N2 fixation by the enzyme nitrogenase occurs under ambient conditions using chemical energy from adenosine 5'-triphosphate (ATP) hydrolysis. We show that cadmium sulfide (CdS) nanocrystals can be used to photosensitize the nitrogenase molybdenum-iron (MoFe) protein, where light harvesting replaces ATP hydrolysis to drive the enzymatic reduction of N2 into NH3 The turnover rate was 75 per minute, 63% of the ATP-coupled reaction rate for the nitrogenase complex under optimal conditions. Inhibitors of nitrogenase (i.e., acetylene, carbon monoxide, and dihydrogen) suppressed N2 reduction. The CdSMoFe protein biohybrids provide a photochemical model for achieving light-driven N2 reduction to NH3.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sulfetos
/
Compostos de Cádmio
/
Molibdoferredoxina
/
Nitrogênio
/
Nitrogenase
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article