Your browser doesn't support javascript.
loading
Glibenclamide enhances the effects of delayed hypothermia after experimental stroke in rats.
Wu, Zhou; Zhu, Shu-Zhen; Hu, Ya-Fang; Gu, Yong; Wang, Sheng-Nan; Lin, Zhen-Zhou; Xie, Zuo-Shan; Pan, Su-Yue.
Afiliação
  • Wu Z; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Zhu SZ; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China; Department of Neurology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
  • Hu YF; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Gu Y; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Wang SN; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Lin ZZ; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Xie ZS; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Pan SY; Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou, China. Electronic address: pansuyue82@126.com.
Brain Res ; 1643: 113-22, 2016 07 15.
Article em En | MEDLINE | ID: mdl-27134036
ABSTRACT
In order to evaluate whether glibenclamide can extend the therapeutic window during which induced hypothermia can protect against stroke, we subjected adult male Sprague-Dawley rats to middle cerebral artery occlusion (MCAO). We first verified the protective effects of hypothermia induced at 0, 2, 4 or 6h after MCAO onset, and then we assessed the effects of the combination of glibenclamide and hypothermia at 6, 8 or 10h after MCAO onset. At 24h after MCAO, we assessed brain edema, infarct volume, modified neurological severity score, Evans Blue leakage and expression of Sulfonylurea receptor 1 (SUR1) protein and pro-inflammatory factors. No protective effects were observed when hypothermia was induced too long after MCAO. At 6h after MCAO onset, hypothermia alone failed to decrease cerebral edema and infarct volume, but the combination of glibenclamide and hypothermia decreased both. The combination also improved neurological outcome, ameliorated blood-brain barrier damage and decreased levels of COX-2, TNF-α and IL-1ß. These results suggest that glibenclamide enhances and extends the therapeutic effects of delayed hypothermia against ischemia stroke, potentially by ameliorating blood-brain barrier damage and declining levels of pro-inflammatory factors.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Edema Encefálico / Glibureto / Fármacos Neuroprotetores / Infarto da Artéria Cerebral Média / Encefalite / Hipertermia Induzida Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Edema Encefálico / Glibureto / Fármacos Neuroprotetores / Infarto da Artéria Cerebral Média / Encefalite / Hipertermia Induzida Tipo de estudo: Etiology_studies Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article