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Melanosomes in pigmented epithelia maintain eye lens transparency during zebrafish embryonic development.
Takamiya, Masanari; Xu, Feng; Suhonen, Heikki; Gourain, Victor; Yang, Lixin; Ho, Nga Yu; Helfen, Lukas; Schröck, Anne; Etard, Christelle; Grabher, Clemens; Rastegar, Sepand; Schlunck, Günther; Reinhard, Thomas; Baumbach, Tilo; Strähle, Uwe.
Afiliação
  • Takamiya M; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Postfach 3640, 76021 Karlsruhe, Germany.
  • Xu F; Institute for Photon Science and Synchrotron Radiation (IPS), Karlsruhe Institute of Technology (KIT), 76021 Karlsruhe, Germany.
  • Suhonen H; European Synchrotron Radiation Facility, 38043 Grenoble, France.
  • Gourain V; University of Helsinki, Department of Physics, 00560 Helsinki, Finland.
  • Yang L; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Postfach 3640, 76021 Karlsruhe, Germany.
  • Ho NY; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Postfach 3640, 76021 Karlsruhe, Germany.
  • Helfen L; Department of Environmental Pollution and Health, State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, 100012 Beijing, China.
  • Schröck A; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Postfach 3640, 76021 Karlsruhe, Germany.
  • Etard C; Institute for Photon Science and Synchrotron Radiation (IPS), Karlsruhe Institute of Technology (KIT), 76021 Karlsruhe, Germany.
  • Grabher C; European Synchrotron Radiation Facility, 38043 Grenoble, France.
  • Rastegar S; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Postfach 3640, 76021 Karlsruhe, Germany.
  • Schlunck G; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Postfach 3640, 76021 Karlsruhe, Germany.
  • Reinhard T; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Postfach 3640, 76021 Karlsruhe, Germany.
  • Baumbach T; Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Postfach 3640, 76021 Karlsruhe, Germany.
  • Strähle U; Eye Center, Freiburg University Medical Center, Killianstr. 5, 79106 Freiburg, Germany.
Sci Rep ; 6: 25046, 2016 05 04.
Article em En | MEDLINE | ID: mdl-27141993
Altered levels of trace elements are associated with increased oxidative stress that is eventually responsible for pathologic conditions. Oxidative stress has been proposed to be involved in eye diseases, including cataract formation. We visualized the distribution of metals and other trace elements in the eye of zebrafish embryos by micro X-ray fluorescence (µ-XRF) imaging. Many elements showed highest accumulation in the retinal pigment epithelium (RPE) of the zebrafish embryo. Knockdown of the zebrafish brown locus homologues tyrp1a/b eliminated accumulation of these elements in the RPE, indicating that they are bound by mature melanosomes. Furthermore, albino (slc45a2) mutants, which completely lack melanosomes, developed abnormal lens reflections similar to the congenital cataract caused by mutation of the myosin chaperon Unc45b, and an in situ spin trapping assay revealed increased oxidative stress in the lens of albino mutants. Finally transplanting a wildtype lens into an albino mutant background resulted in cataract formation. These data suggest that melanosomes in pigment epithelial cells protect the lens from oxidative stress during embryonic development, likely by buffering trace elements.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Melanossomas / Desenvolvimento Embrionário / Cristalino Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Melanossomas / Desenvolvimento Embrionário / Cristalino Limite: Animals Idioma: En Ano de publicação: 2016 Tipo de documento: Article