Application of Goldmag immune probe in timely detection of syphilis based on GIS platform.
Artif Cells Nanomed Biotechnol
; 45(3): 460-466, 2017 May.
Article
em En
| MEDLINE
| ID: mdl-27152908
ABSTRACT
The purpose of this study was to apply goldmag immunoprobes into establishment of nanoparticles-based colorimetric assay as well as construction of immunochromatography quantitative and qualitative system by exploring point-of-care testing of syphilis with goldmag particles carrier-based immunoprobe and analysis of spatial data of Geographic Information System (GIS) platform. Goat anti-rabbit immunoglobulin G (IgG) was coupled on the surface of modified nanoparticles, taking N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide as the connector. Then the nanoparticles were used for colorimetric detection of goat-anti-rabbit IgG in liquid phase system. Based on the analysis of spatial data in GIS platform, we found the probe constructed based on MUA-Fe304/Au nanoparticles responded more sensitive to detection objects compared with the probe designed based on PAA-Fe3O4/Au nanoparticles, and its reaction rate constant was two times that of PAA-Fe3O4/Au nanoparticles based goldmag immunoprobe. Goldmag particles not only can be coupled with biomolecules such as antibody/antigen and glycoprotein but also possess superparamagnetism.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Treponema pallidum
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Sondas Moleculares
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Sífilis
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Cromatografia de Afinidade
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Nanopartículas de Magnetita
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Anticorpos Antibacterianos
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Antígenos de Bactérias
Tipo de estudo:
Diagnostic_studies
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Qualitative_research
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article