Development of a novel engineered antibody targeting human CD123.
Anal Biochem
; 511: 27-30, 2016 10 15.
Article
em En
| MEDLINE
| ID: mdl-27156812
ABSTRACT
Antibody engineering involves a range of custom modifications of immunoglobulins to improve their affinity, valency, and pharmacokinetics, ensuring a better target therapy achievement. A number of therapeutic antibodies have been used for cell surface receptor blockage, interfering with the ligand binding and inhibiting receptor-driven activation of cells. Here we describe the construction and characterization of a recombinant bivalent single-chain Fv (biscFv) that targets CD123. On conversion of anti-CD123 scFv to biscFv format, the recognition of the cognate ligand is not altered. Moreover, the increased overall efficacy of the anti-CD123 biscFv in binding and inhibition of CD123/IL-3 (interleukin-3) interactions in TF-1 cells is demonstrated.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Anticorpos Biespecíficos
/
Subunidade alfa de Receptor de Interleucina-3
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Anticorpos de Cadeia Única
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article