Immunomodulatory Properties of Induced Pluripotent Stem Cell-Derived Mesenchymal Cells.
J Cell Biochem
; 117(12): 2844-2853, 2016 12.
Article
em En
| MEDLINE
| ID: mdl-27167148
ABSTRACT
MSC-like populations derived from induced pluripotent stem cells (iPSC-MSC) serve as an alternative stem cell source due to their high proliferative capacity. In this study, we assessed the immunomodulatory potential of iPSC-MSC generated from periodontal ligament (PDL) and gingival (GF) tissue. The iPSC-MSC lines exhibited a similar level of suppression of mitogen-stimulated peripheral blood mononuclear cells (PBMNC) proliferation compared to their respective parental fibroblast populations in vitro. Moreover, iPSC-MSC demonstrated the ability to suppress T-cells effector cells, Th1/Th2/Th17 populations, and increase levels of Treg cells. In order to investigate the mechanisms involved, expression of common MSC-derived soluble factors known to supress lymphocyte proliferation were assessed in iPSC-MSC cultured with PBMNC with direct cell-cell contact or separated in transwells. Real-time PCR analysis of factors known to be involved in MSC mediated immune regulation, found a general trend of elevated IDO1 and IL6 transcript levels in iPSC-MSC lines and their respective primary cells co-cultured with activated PBMNC, with a wide range of gene expression levels between the different mesenchymal cell types. The results suggest that different iPSC-MSC may be useful as a potential alternative source of cells for future clinical use in therapeutic applications because of their potent immunosuppressive properties. J. Cell. Biochem. 117 2844-2853, 2016. © 2016 Wiley Periodicals, Inc.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ligamento Periodontal
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Leucócitos Mononucleares
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Linfócitos T Reguladores
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Células-Tronco Pluripotentes Induzidas
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Células-Tronco Mesenquimais
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Gengiva
Limite:
Humans
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article