Development and validation of an HILIC-MS/MS method by one-step precipitation for chloroquine in miniature pig plasma.
Bioanalysis
; 8(11): 1159-71, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-27211608
ABSTRACT
BACKGROUND:
Quantification of polar compounds such as chloroquine by revered-phase LC is a challenge because of poor retention and silanol interactions with stationary phase. Strong ion-pairing reagents added to mobile phases to improve reversed-phase retention and improve peak shape can be harmful for MS.RESULTS:
This new approach provides a rapid and sensitive method for the detection of chloroquine using hydrophilic interaction LC coupled to MS/MS (HILIC-MS/MS). Ammonium formate and formic acid were added to mobile phase to attain good peak shapes and the salified chloroquine as well retained in an HILIC column. Linearity, intra- and inter-day precision, accuracy, recovery, matrix effect and stability were evaluated during the validation process.CONCLUSION:
The validated method has been successfully used in a PK study in miniature pigs, and paves way for future development.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Porco Miniatura
/
Cloroquina
/
Espectrometria de Massas em Tandem
/
Antimaláricos
Limite:
Animals
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article