Reliable transformation system for Microbotryum lychnidis-dioicae informed by genome and transcriptome project.
Arch Microbiol
; 198(8): 813-25, 2016 Oct.
Article
em En
| MEDLINE
| ID: mdl-27215216
ABSTRACT
Microbotryum lychnidis-dioicae is a member of a species complex infecting host plants in the Caryophyllaceae. It is used as a model system in many areas of research, but attempts to make this organism tractable for reverse genetic approaches have not been fruitful. Here, we exploited the recently obtained genome sequence and transcriptome analysis to inform our design of constructs for use in Agrobacterium-mediated transformation techniques currently available for other fungi. Reproducible transformation was demonstrated at the genomic, transcriptional and functional levels. Moreover, these initial proof-of-principle experiments provide evidence that supports the findings from initial global transcriptome analysis regarding expression from the respective promoters under different growth conditions of the fungus. The technique thus provides for the first time the ability to stably introduce transgenes and over-express target M. lychnidis-dioicae genes.
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Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
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Plantas
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Transformação Genética
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Basidiomycota
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Genoma Fúngico
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Técnicas de Transferência de Genes
Tipo de estudo:
Prognostic_studies
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article