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Comparison of Two Widely Used Human Papillomavirus Detection and Genotyping Methods, GP5+/6+-Based PCR Followed by Reverse Line Blot Hybridization and Multiplex Type-Specific E7-Based PCR.
Clifford, Gary M; Vaccarella, Salvatore; Franceschi, Silvia; Tenet, Vanessa; Umulisa, M Chantal; Tshomo, Ugyen; Dondog, Bolormaa; Vorsters, Alex; Tommasino, Massimo; Heideman, Daniëlle A M; Snijders, Peter J F; Gheit, Tarik.
Afiliação
  • Clifford GM; International Agency for Research on Cancer, Lyon, France clifford@iarc.fr.
  • Vaccarella S; International Agency for Research on Cancer, Lyon, France.
  • Franceschi S; International Agency for Research on Cancer, Lyon, France.
  • Tenet V; International Agency for Research on Cancer, Lyon, France.
  • Umulisa MC; Ministry of Health of Rwanda, Kigali, Rwanda.
  • Tshomo U; Department of Obstetrics and Gynaecology, Jigme Dorji Wangchuck National Referral Hospital, Thimphu, Bhutan.
  • Dondog B; Infection and Cancer Program, German Cancer Research Center, Heidelberg, Germany.
  • Vorsters A; Centre for the Evaluation of Vaccination, Vaccine and Infectious Disease Institute, University of Antwerp, Antwerp, Belgium.
  • Tommasino M; International Agency for Research on Cancer, Lyon, France.
  • Heideman DA; Department of Pathology, VU University Medical Center, Amsterdam, the Netherlands.
  • Snijders PJ; Department of Pathology, VU University Medical Center, Amsterdam, the Netherlands.
  • Gheit T; International Agency for Research on Cancer, Lyon, France.
J Clin Microbiol ; 54(8): 2031-8, 2016 08.
Article em En | MEDLINE | ID: mdl-27225411
GP5+/6+-based PCR followed by reverse line blot hybridization (GP5+/6+RLB) and multiplex type-specific PCR (E7-MPG) are two human papillomavirus (HPV) genotyping methodologies widely applied in epidemiological research. We investigated their relative analytical performance in 4,662 samples derived from five studies in Bhutan, Rwanda, and Mongolia coordinated by the International Agency for Research on Cancer (IARC). A total of 630 samples were positive by E7-MPG only (13.5%), 24 were positive by GP5+/6+RLB only (0.5%), and 1,014 were positive (21.8%) by both methods. Ratios of HPV type-specific positivity of the two tests (E7-MPG:GP5+/6+RLB ratio) were calculated among 1,668 samples that were HPV positive by one or both tests. E7-MPG:GP5+/6+RLB ratios were >1 for all types and highly reproducible across populations and sample types. E7-MPG:GP5+/6+RLB ratios were highest for HPV53 (7.5) and HPV68 (7.1). HPV16 (1.6) and HPV18 (1.7) had lower than average E7-MPG:GP5+/6+RLB ratios. Among E7-MPG positive infections, median mean fluorescence intensity (MFI; a semiquantitative measure of viral load) tended to be higher among samples positive for the same virus type by GP5+/6+RLB than for those negative for the same type by GP5+/6+RLB. Exceptions, however, included HPV53, -59, and -82, for which the chances of being undetected by GP5+/6+RLB appeared to be MFI independent. Furthermore, the probability of detecting an additional type by E7-MPG was higher when another type was already detected by GP5+/6+RLB, suggesting the existence of masking effects due to competition for GP5+/6+ PCR primers. In conclusion, this analysis is not an evaluation of clinical performance but may inform choices for HPV genotyping methods in epidemiological studies, when the relative merits and dangers of sensitivity versus specificity for individual types should be considered, as well as the potential to unmask nonvaccine types following HPV vaccination.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Papillomaviridae / Infecções por Papillomavirus / Técnicas de Diagnóstico Molecular / Técnicas de Genotipagem / Genótipo Tipo de estudo: Diagnostic_studies Limite: Adolescent / Adult / Aged / Female / Humans / Middle aged País como assunto: Africa / Asia Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Papillomaviridae / Infecções por Papillomavirus / Técnicas de Diagnóstico Molecular / Técnicas de Genotipagem / Genótipo Tipo de estudo: Diagnostic_studies Limite: Adolescent / Adult / Aged / Female / Humans / Middle aged País como assunto: Africa / Asia Idioma: En Ano de publicação: 2016 Tipo de documento: Article