An LC-MRM method for measuring intestinal triglyceride assembly using an oral stable isotope-labeled fat challenge.
Bioanalysis
; 8(12): 1265-77, 2016 Jun.
Article
em En
| MEDLINE
| ID: mdl-27225968
ABSTRACT
AIM:
A traditional oral fatty acid challenge assesses absorption of triacylglycerol (TG) into the periphery through the intestines, but cannot distinguish the composition or source of fatty acid in the TG. Stable isotope-labeled tracers combined with LC-MRM can be used to identify and distinguish TG synthesized with dietary and stored fatty acids.RESULTS:
Concentrations of three abundant TGs (522, 543 and 544) were monitored for incorporation of one or two (2)H11-oleate molecules per TG. This method was subjected to routine assay validation and meets typical requirements for an assay to be used to support clinical studies.CONCLUSION:
Calculations for the fractional appearance rate of TG in plasma are presented along with the intracellular enterocyte precursor pool for 12 study participants.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Triglicerídeos
/
Cromatografia Líquida de Alta Pressão
/
Mucosa Intestinal
Limite:
Adolescent
/
Adult
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Humans
/
Male
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article