Differentiation of rinderpest and peste des petits ruminants viruses using specific cDNA clones.
J Virol Methods
; 23(2): 127-36, 1989 Feb.
Article
em En
| MEDLINE
| ID: mdl-2723018
ABSTRACT
The morbilliviruses which infect ruminants, rinderpest (RPV) and peste des petits ruminants (PPRV), are difficult to distinguish serologically. They can be distinguished by differential neutralisation tests and by the migration of the major virus structural protein, the nucleocapsid protein, on polyacrylamide gels. Both these methods are time consuming and require the isolation of live virus for identification; they are not suitable for analysis of material directly from post-mortem specimens. We describe a rapid method for differential diagnosis of infections caused by RPV or PPRV, which uses specific cDNA probes, derived from the mRNAs for the nucleocapsid protein of each virus, which can be used to distinguish unequivocally the two virus types rapidly.
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Base de dados:
MEDLINE
Assunto principal:
Peste Bovina
/
Vírus da Peste Bovina
/
DNA Viral
/
Sondas de DNA
/
Proteínas do Core Viral
/
Capsídeo
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
1989
Tipo de documento:
Article