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Sphingomyelin is sorted at the trans Golgi network into a distinct class of secretory vesicle.
Deng, Yongqiang; Rivera-Molina, Felix E; Toomre, Derek K; Burd, Christopher G.
Afiliação
  • Deng Y; Department of Cell Biology, Yale School of Medicine, New Haven, CT 06520.
  • Rivera-Molina FE; Department of Cell Biology, Yale School of Medicine, New Haven, CT 06520.
  • Toomre DK; Department of Cell Biology, Yale School of Medicine, New Haven, CT 06520.
  • Burd CG; Department of Cell Biology, Yale School of Medicine, New Haven, CT 06520 christopher.burd@yale.edu.
Proc Natl Acad Sci U S A ; 113(24): 6677-82, 2016 06 14.
Article em En | MEDLINE | ID: mdl-27247384
ABSTRACT
One of the principal functions of the trans Golgi network (TGN) is the sorting of proteins into distinct vesicular transport carriers that mediate secretion and interorganelle trafficking. Are lipids also sorted into distinct TGN-derived carriers? The Golgi is the principal site of the synthesis of sphingomyelin (SM), an abundant sphingolipid that is transported. To address the specificity of SM transport to the plasma membrane, we engineered a natural SM-binding pore-forming toxin, equinatoxin II (Eqt), into a nontoxic reporter termed Eqt-SM and used it to monitor intracellular trafficking of SM. Using quantitative live cell imaging, we found that Eqt-SM is enriched in a subset of TGN-derived secretory vesicles that are also enriched in a glycophosphatidylinositol-anchored protein. In contrast, an integral membrane secretory protein (CD8α) is not enriched in these carriers. Our results demonstrate the sorting of native SM at the TGN and its transport to the plasma membrane by specific carriers.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Esfingomielinas / Membrana Celular / Antígenos CD8 / Vesículas Secretórias / Rede trans-Golgi Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Esfingomielinas / Membrana Celular / Antígenos CD8 / Vesículas Secretórias / Rede trans-Golgi Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article