Application of arteether-loaded polyurethane nanomicelles to induce immune response in breast cancer model.
Artif Cells Nanomed Biotechnol
; 45(4): 808-816, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-27263545
ABSTRACT
To concentrate a potent anticancer drug (Arteether) in tumor microenvironment, we encapsulated it in biodegradable and pH sensitive polyurethane (PU) nanomicelles (NMs). The nanocomplex was characterized by Fourier transform infrared (FTIR), dynamic light scattering (DLS). The loading capacity and release profile in pH of 5.4 and 7.4 were considered. The cytotoxicity effect was evaluated in vitro and in vivo settings. The level of IFN-γ and IL-4 cytokines of mice splenocytes were assessed by enzyme-linked immunosorbent assay (ELISA). The nanocomplex showed negative zeta charge of -26.2 mV, size of 42.30 nm and high loading capacity (92%). Release profile showed a faster rate of drug liberation at pH 5.4 as compared to that of pH 7.4. It indicated significant inhibitory effect on the growth of 4T1 cell line and increased IFN-γ level.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Poliuretanos
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Neoplasias da Mama
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Portadores de Fármacos
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Artemisininas
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Nanopartículas
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Neoplasias Mamárias Experimentais
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Micelas
Limite:
Animals
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article