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Establishment and characterization of a highly immunogenic human renal carcinoma cell line.
Prattichizzo, Clelia; Gigante, Margherita; Pontrelli, Paola; Stella, Alessandro; Rocchetti, Maria Teresa; Gigante, Maddalena; Maiorano, Eugenio; Herr, Wolfgang; Battaglia, Michele; Gesualdo, Loreto; Ranieri, Elena.
Afiliação
  • Prattichizzo C; Department of Medical and Surgical Sciences, Section of Clinical Pathology, University of Foggia, Foggia, Italy.
  • Gigante M; Department of Emergency and Organ Transplantation, Section of Nephrology, University of Bari 'Aldo Moro', Bari, Italy.
  • Pontrelli P; Department of Emergency and Organ Transplantation, Section of Nephrology, University of Bari 'Aldo Moro', Bari, Italy.
  • Stella A; Medical Genetics Unit, Department of Biomedicine in Childhood, University of Bari 'Aldo Moro', Bari, Italy.
  • Rocchetti MT; Department of Emergency and Organ Transplantation, Section of Nephrology, University of Bari 'Aldo Moro', Bari, Italy.
  • Gigante M; Department of Medical and Surgical Sciences, Section of Clinical Pathology, University of Foggia, Foggia, Italy.
  • Maiorano E; Department of Pathological Anatomy, University of Bari 'Aldo Moro', Bari, Italy.
  • Herr W; Department of Medicine III, Johannes Gutenberg-University Mainz, Mainz, Germany.
  • Battaglia M; Section of Urology, University of Bari 'Aldo Moro', Bari, Italy.
  • Gesualdo L; Department of Emergency and Organ Transplantation, Section of Nephrology, University of Bari 'Aldo Moro', Bari, Italy.
  • Ranieri E; Department of Medical and Surgical Sciences, Section of Clinical Pathology, University of Foggia, Foggia, Italy.
Int J Oncol ; 49(2): 457-70, 2016 Aug.
Article em En | MEDLINE | ID: mdl-27278998
ABSTRACT
Renal cell carcinoma (RCC) is the most common kidney cancer, and accounts for ~3% of all adult malignancies. RCC has proven refractory to conventional treatment modalities but appears to be the only histological form that shows any consistent response to immunotherapeutic approaches. The development of a clinically effective vaccine remains a major strategic target for devising active specific immunotherapy in RCC. We aimed to identify a highly immunogenic antigenic format for immunotherapeutic approaches, so as to boost immune responses in RCC patients. We established and cloned an immunogenic cell line, RCC85#21 named Elthem, which was derived from a non-aggressive and non-metastatic clear cell carcinoma. The cell line characterization was performed by genomics (real-time PCR, genome instability), proteomics (two dimensional electrophoresis, mass spectro-metry) and immunological analysis (mixed lymphocytes tumor cell cultures). Real-time PCR confirmed the RCC85#21 cell expression of tumor antigens and cytokine genes. No difference in microsatellite instability (MSI) in RCC85#21 cell line was found as compared to control, loss of heterozygosity was observed in the RCC85#21 clone, but not in the renal cancer cell lines from which it was generated. The image analysis of RCC85#21 by two-dimensional gels showed 700±26 spots and 119 spots were identified by mass spectrometry analysis. RCC85#21 promoted a significant RCC-specific T cells activation by exhibiting a cytotoxic phenotype after mixed lymphocyte and tumor cell cultures. CD8+ T cells isolated from RCC patients displayed an elevated reactivity against RCC85#21 and efficiently lysed the RCC85#21 clone. The RCC85#21 immunogenic cell line will be suitable for immune stimulation. The identification of novel tumor associated antigens will allow the evaluation of the immune response in vitro and, subsequently, in vivo paving the way for new immunotherapeutic strategies in the RCC setting.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carcinoma de Células Renais / Linhagem Celular Tumoral Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carcinoma de Células Renais / Linhagem Celular Tumoral Limite: Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article