The internalization of Helicobacter pylori plays a role in the failure of H. pylori eradication.
Helicobacter
; 22(1)2017 Feb.
Article
em En
| MEDLINE
| ID: mdl-27282442
ABSTRACT
BACKGROUND:
Helicobacter pylori (H. pylori) internalization involves invasion of cells by the bacterium. Several studies have shown that H. pylori can invade human gastric epithelial cells, immune cells, and Candida yeast in vivo and in vitro. Whether bacterial invasion plays a role in eradication failure is unclear.AIM:
To investigate the relationship between H. pylori invasion of GES-1 cells and H. pylori eradication failure. MATERIALS ANDMETHODS:
Forty-two clinical strains isolated from H. pylori-positive patients with different outcomes after treatment with furazolidone-based therapy were examined (17 failures and 25 successes). The H. pylori strains were shown to be susceptible to amoxicillin and furazolidone, and the patients also exhibited good compliance. Genotyping was performed for cagA and vacA (s and m). The antibiotic susceptibility of the strains to amoxicillin, furazolidone, clarithromycin, metronidazole, and levofloxacin was determined by E-tests. The levels of H. pylori invasion of GES-1 cells were detected by gentamicin colony-forming unit assays.RESULTS:
The internalization level in the eradication success group was 5.40±5.78 × 10-3 cfu/cell, and the median was 6.194 × 10-3 cfu/cell; the internalization level in the eradication failure group was 8.98±5.40 × 10-3 cfu/cell, and the median was 10.28 × 10-3 cfu/cell. The eradication failure group showed a greater invasion level than the eradication success group (P<.05). No significant difference was observed between the susceptible strains and the resistant strains when the internalization levels were compared (P>.05).CONCLUSIONS:
The results showed that H. pylori invasion of the gastric epithelia might play a role in eradication failure.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Helicobacter pylori
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Infecções por Helicobacter
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Endocitose
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Células Epiteliais
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Interações Hospedeiro-Patógeno
Limite:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Ano de publicação:
2017
Tipo de documento:
Article