Endoplasmic reticulum stress in mouse decidua during early pregnancy.
Mol Cell Endocrinol
; 434: 48-56, 2016 10 15.
Article
em En
| MEDLINE
| ID: mdl-27283502
ABSTRACT
Unfolded or misfolded protein accumulation in the endoplasmic reticulum lumen leads to endoplasmic reticulum stress (ER stress). Although it is known that ER stress is crucial for mammalian reproduction, little is known about its physiological significance and underlying mechanism during decidualization. Here we show that Ire-Xbp1 signal transduction pathway of unfolded protein response (UPR) is activated in decidual cells. The process of decidualization is compromised by ER stress inhibitor tauroursodeoxycholic acid sodium (TUDCA) and Ire specific inhibitor STF-083010 both in vivo and in vitro. A high concentration of ER stress inducer tunicamycin (TM) suppresses stromal cells proliferation and decidualization, while a lower concentration is beneficial. We further show that ER stress induces DNA damage and polyploidization in stromal cells. In conclusion, our data suggest that the GRP78/Ire1/Xbp1 signaling pathway of ER stress-UPR is activated and involved in mouse decidualization.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Serina-Treonina Quinases
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Decídua
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Endorribonucleases
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Estresse do Retículo Endoplasmático
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Proteína 1 de Ligação a X-Box
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Proteínas de Choque Térmico
Limite:
Animals
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Pregnancy
Idioma:
En
Ano de publicação:
2016
Tipo de documento:
Article