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Neuroglobin overexpression induced by the 17ß-Estradiol-Estrogen receptor-α Pathway reduces the sensitivity of MCF-7 Breast cancer cell to paclitaxel.
Fiocchetti, Marco; Cipolletti, Manuela; Leone, Stefano; Ascenzi, Paolo; Marino, Maria.
Afiliação
  • Fiocchetti M; Department of Science, Roma Tre University, Roma, Italy.
  • Cipolletti M; Department of Science, Roma Tre University, Roma, Italy.
  • Leone S; Department of Science, Roma Tre University, Roma, Italy.
  • Ascenzi P; Department of Science, Roma Tre University, Roma, Italy.
  • Marino M; Interdepartmental Laboratory of Electron Microscopy, Roma Tre University, Roma, Italy.
IUBMB Life ; 68(8): 645-51, 2016 08.
Article em En | MEDLINE | ID: mdl-27312786
Although paclitaxel (Taxol) is an active chemotherapeutic agent for the treatment of breast cancer, not all breast tumors are sensitive to this drug. In particular, there is a wide agreement on the low sensitivity of estrogen receptor (ER) α-positive breast cancer to paclitaxel treatment. However, the ERα-based insensitivity to paclitaxel is still elusive. Here, the effect of the E2/ERα-dependent upregulation of neuroglobin (NGB), an antiapoptotic globin, on the reduced sensitivity of breast cancer cells to paclitaxel-induced apoptosis has been evaluated in ERα-containing MCF-7 cells. The E2 pretreatment enhances the ERα activity and significantly impairs paclitaxel-induced apoptosis as evaluated by Annexin V assay and PARP-1 cleavage. NGB displays a pivotal role in the E2/ERα-induced antiapoptotic pathway to abrogate paclitaxel-induced cell death in stable NGB-silenced MCF-7 cell clones. Moreover, in the absence of the active ERα, paclitaxel significantly reduces the NGB cell content. In conclusion, these results highlight the involvement of ERα activation and of E2/ERα-dependent NGB upregulation in the insensitivity of MCF-7 to paclitaxel. These novel findings could have important implications in the development of targeted therapeutics for overcoming paclitaxel insensitivity in ERα-positive human breast cancer. © 2016 IUBMB Life, 68(8):645-651, 2016.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Globinas / Resistencia a Medicamentos Antineoplásicos / Receptor alfa de Estrogênio / Proteínas do Tecido Nervoso Tipo de estudo: Diagnostic_studies Limite: Female / Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Globinas / Resistencia a Medicamentos Antineoplásicos / Receptor alfa de Estrogênio / Proteínas do Tecido Nervoso Tipo de estudo: Diagnostic_studies Limite: Female / Humans Idioma: En Ano de publicação: 2016 Tipo de documento: Article